SENSOCELL +

SENSOCELL Optical Tweezers

The only optical tweezers platform offering calibration-free force
measurements and integrated high-speed confocal imaging for correlated
quantitative assays from single molecules to cells and living organisms.

01 / KEY FEATURES

SENSOCELL+

Integrated Confocal imaging

Correlated high-speed fluorescence imaging with force and position data

Measure across scales

From single-molecule assays to intracellular mechanics and in vivo force measurements.

Calibration-free force measurements

Measure forces directly without prior trap-stiffness calibration.

Programable multitrap
control

Generate up to 256 traps and automate trajectories, oscillations and force-measurement routines.

02 / APPLICATIONS

One platform
Multiple research scales

Explore quantitative assays across molecules, cells, living systems and soft matter.

MECHANOBIOLOGY

Measure forces and viscoelasticity to investigate mechanotransduction in cells, tissues and living organisms.

MOLECULAR BIOPHYSICS

Explore single-molecule dynamics by quantifying forces and interactions.

BIOMOLECULAR CONDENSATES

Investigate the mechanics, viscoelasticity and fusion dynamics of biomolecular condensates.

SOFT MATTER PHYSICS

Characterize the mechanical properties of colloids and active systems through force probing and microrheology.

03 / LIGHTACE SOFTWARE

Ready. Set
Measure

LightACE brings automated multitrap control, real-time force measurement, built-in experimental workflows and synchronized recording of video, force and position data into a single control environment.

LIghtAce integrates seamlessly with PhaseStreak Confocal SharpEye software.

INTUITIVE INTERACTIVE CONTROL

Create, select and move optical traps directly through the live camera view, and configure measurements and routines using intuitive menus.

PROGRAMMABLE MULTITRAP CONTROL

Generate and independently control up to 256 optical traps, and program trajectories, oscillations, and dynamic pattern sequences.

REAL-TIME FORCE CONTROL

Monitor force and position data in real time and run feedback-controlled force-clamp routines on multiple traps.

AUTOMATED ACTIVE MICRORHEOLOGY

Run automated active microrheology assays and quantify local stiffness, viscosity and elasticity with the integrated TimSOM workflow.

SYNCHRONIZED DATA ACQUISITION

Record experimental videos synchronized with force and position data on a common timeline.

CUSTOM ROUTINES AND ANALYSIS

Access a growing library of assay-specific data-analysis plugins. Build custom experimental routines with the visual LightACE SDK.

04 / SYSTEM CONFIGURATION

Start with your microscope
Or start turnkey

SENSOCELL+ is a modular optical tweezers platform with integrated options for confocal imaging and live-cell incubation. Built around the SENSOCELL optical tweezers system, it is available as an add-on to a compatible microscope or as a complete turnkey system.

ADD-ON CONFIGURATION

Integrate SENSOCELL+ with a compatible Nikon Ti or Ti2 microscope already available in your laboratory.

SENSOCELL OPTICAL TWEEZERS

  • Programmable optical manipulation
  • Calibration-free force detection
  • LightACE control software
  • 3 W, 1064 nm trapping laser

 

OPTIONAL INTEGRATED MODULES

  • PhaseStreak high-speed confocal imaging
  • Live-cell incubation

TURNKEY CONFIGURATION

A complete optical tweezers workstation, assembled and configured around your experimental requirements.

COMPLETE WORKSTATION

  • SENSOCELL optical tweezers
  • Nikon Ti2 inverted microscope
  • Scientific imaging camera
  • LightACE software and control workstation

 

OPTIONAL INTEGRATED MODULES

  • PhaseStreak high-speed confocal imaging
  • Live-cell incubation
05 / KEY PERFORMANCE

Values

Calibration-free
DIRECT FORCE MEASUREMENT
< 50 fN
FORCE RESOLUTION
> 500 pN
MAXIMUM APPLIED FORCE
< 10%
TYPICAL ACCURACY IN CELLS
Up to 256
INDEPENDENT TRAPS & SIMULTANEOUS FORCE READOUT
25 kHz
TRAP STEERING & FORCE SAMPLING
Up to 230 fps
FULL-FOV CONFOCAL IMAGING *
Up to 42 ºC
LIVE-CELL INCUBATION **

* Available with the optional integrated PhaseStreak Confocal module.

** Available with the optional integrated live-cell incubation module.

06 / EXPLORE WHAT’S POSSIBLE

Build the experiment you envision

Tell us about your samples, assays and imaging needs. We’ll work with you to define the right SENSOCELL+ configuration for your research

WHY IMPETUX

What sensocell
users say

“

Fig. 1

Time dependence of trap position (top), force signal (middle) and Ca2+ fluorescence signal (bottom) recorded during a membrane tether pulling experiment. The force and Ca2+ ion channel activity signals exhibit negative correlation.

Fig. 2

Force and displacement data for two beads stimulated at frequencies of 4 Hz and 0.25 Hz. The progressive reduction of the beads displacement is in concomitancy with an increase of the applied force and loading rate.

Fig. 1

Schematic representation of the optical tweezers experiment. A fibronectin-coated bead is trapped, brought into contact with the cell membrane and stimulated with oscillations at different frequencies.

Fig.2

Time dependence of trap 1 and trap 2 position (top) and force (bottom) data along the different steps of the described dual tether pulling experiment.

Fig.1

Confocal video of the described dual tether pulling assay performed on a neuron axon. Courtesy of M. Krieg lab (ICFO).

Fig. 1

Time dependence of trap position (top), force signal (middle) and Ca2+ fluorescence signal (bottom) recorded during a membrane tether pulling experiment. The force and Ca2+ ion channel activity signals exhibit negative correlation.

Video 1

Confocal video showing a DVA neuron Ca2+ ion channel activity in a dynamic optical trapping assay. The pulling rate applied to the membrane tether is progressively increased. Scale bar = 5µm. Acquired at 10Hz.