BLOG

Sensing force from outside and inside cells | EMBO Workshop

Sensing force from outside and inside cells | EMBO Workshop

EMBO workshop: Physics of living systems: From molecules to tissues

📅DATE: June 07-11, 2021

The next event in which IMPETUX will be present will be virtual and will be the EMBO workshop:  Physics of living systems: From molecules to tissues

We will be participating with a talk to show how our patented cutting-edge technology can be a fundamental tool to reveal new understanding in this amazing field.

📅June 17, at 14:40 h. CET  we will be on line with the talk:

SENSOCELL Optical tweezers: sensing force from outside and inside cells

A great opportunity to meet comfortably for discussion and feedback!.

You can head over to the event website to check out the program and register here.

📌If you are interested in how our optical tweezers system: SENSOCELL can push your research forward in applications such as the measurement of cell membrane forces, the study of nucleus mechanics, the measurement of cell-cell interaction forces among others, do not hesitate to join us or contact us any time!

 

Time remaining before the start of this event:

More news:

Fig. 1

Time dependence of trap position (top), force signal (middle) and Ca2+ fluorescence signal (bottom) recorded during a membrane tether pulling experiment. The force and Ca2+ ion channel activity signals exhibit negative correlation.

Fig. 2

Force and displacement data for two beads stimulated at frequencies of 4 Hz and 0.25 Hz. The progressive reduction of the beads displacement is in concomitancy with an increase of the applied force and loading rate.

Fig. 1

Schematic representation of the optical tweezers experiment. A fibronectin-coated bead is trapped, brought into contact with the cell membrane and stimulated with oscillations at different frequencies.

Fig.2

Time dependence of trap 1 and trap 2 position (top) and force (bottom) data along the different steps of the described dual tether pulling experiment.

Fig.1

Confocal video of the described dual tether pulling assay performed on a neuron axon. Courtesy of M. Krieg lab (ICFO).

Fig. 1

Time dependence of trap position (top), force signal (middle) and Ca2+ fluorescence signal (bottom) recorded during a membrane tether pulling experiment. The force and Ca2+ ion channel activity signals exhibit negative correlation.

Video 1

Confocal video showing a DVA neuron Ca2+ ion channel activity in a dynamic optical trapping assay. The pulling rate applied to the membrane tether is progressively increased. Scale bar = 5µm. Acquired at 10Hz.