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Sensing Membranes with Optical Tweezers | EMBO Workshop

Sensing Membranes with Optical Tweezers | EMBO Workshop

EMBO workshop: Cell polarity and membrane dynamics

📅DATE: May 16-21, 2021

The next event in which IMPETUX will be present will be virtual and will be the EMBO workshop: Cell polarity and membrane dynamics.

This EMBO Workshop will explore the intersection between polarity, cytoskeleton and cellular membrane networks in the context of polarized cells and tissues. It will bring together researchers from around the globe and across career stages to identify and highlight new principles of cellular organization and how these concepts impact our understanding of the role of polarity and membrane dynamics in human development and disease.

We will be participating with a talk and a Q&A session to show how our patented cutting-edge technology can be a fundamental tool to reveal new understanding in this amazing field.

The talk will be 🎥 pre-recorded and made available to registered participants.

📅May 17, at 15:30 h. CET  we will be on a live discussion session to answer all your questions.

A great opportunity to meet comfortably for discussion and feedback!

You can head over to the event website to check out the program and register here.

📌If you are interested in how our optical tweezers system: SENSOCELL can push your research forward in applications such as the measurement of cell membrane forces, the study of nucleus mechanics, the measurement of cell-cell interaction forces among others, do not hesitate to join us or contact us any time!

 

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Fig. 1

Time dependence of trap position (top), force signal (middle) and Ca2+ fluorescence signal (bottom) recorded during a membrane tether pulling experiment. The force and Ca2+ ion channel activity signals exhibit negative correlation.

Fig. 2

Force and displacement data for two beads stimulated at frequencies of 4 Hz and 0.25 Hz. The progressive reduction of the beads displacement is in concomitancy with an increase of the applied force and loading rate.

Fig. 1

Schematic representation of the optical tweezers experiment. A fibronectin-coated bead is trapped, brought into contact with the cell membrane and stimulated with oscillations at different frequencies.

Fig.2

Time dependence of trap 1 and trap 2 position (top) and force (bottom) data along the different steps of the described dual tether pulling experiment.

Fig.1

Confocal video of the described dual tether pulling assay performed on a neuron axon. Courtesy of M. Krieg lab (ICFO).

Fig. 1

Time dependence of trap position (top), force signal (middle) and Ca2+ fluorescence signal (bottom) recorded during a membrane tether pulling experiment. The force and Ca2+ ion channel activity signals exhibit negative correlation.

Video 1

Confocal video showing a DVA neuron Ca2+ ion channel activity in a dynamic optical trapping assay. The pulling rate applied to the membrane tether is progressively increased. Scale bar = 5µm. Acquired at 10Hz.